8324_onestep_probe_multiplex.jpg

Some applications of this product  may require a license which is not provided by the purchase of this product.

For research use only. 

Sensitive and highly specific cDNA synthesis and qPCR in a single tube

Convenient kit format for performing highly specific cDNA synthesis and probe-based qPCR in a single tube. The kit contains qPCR mix, SOLIScript® RT enzyme mix with RiboGrip™ RNase inhibitor, and nuclease-free water.

  • cDNA synthesis up to 60°C for superior specificity
  • analyze 1-4 targets in 1 reaction
  • reaction set-up and shipment without dry ice
  • robust amplification of GC-rich targets
  • contains dUTP to prevent cross-contamination when used in combination with UNG
  • RNase inhibitor included in kit

Ordering

Choose Mix
Choose Product Size
Catalog number

08-55-0000S

Quantity

€ 0.00

Log in to see Your Price

Details

Reagents Provided
Item Pcs. Vial size
40x One-step SOLIScript® Mix 1 50 rxn | 25 µl
5x One-step Multiplex Probe Mix 1 50 rxn | 200 µl
Water, nuclease free 1 1.25 ml
Overview

Description

SOLIScript® 1-step Multiplex Probe Kit is optimized for probe based one-step RT real-time quantitative PCR assays. It contains all the components necessary (except template, primers, and probes) to perform cDNA synthesis and probe based qPCR in a single tube.

Reagent composition is optimized for excellent performance in highly multiplexed reactions while making sure you don't lose in sensitivity compared to singleplex or duplex assays.

Applications

  • Detection and quantification of RNA targets with qPCR
  • DNA/LNA hydrolysis probe based assays
  • Suitable for singleplex and multiplex reactions

Properties

Sample type: RNA

Product format: Reagent kit 

Detection type: Probe-based

Reference dye: none

Compatible real-time instruments: The kit is compatible with qPCR cyclers that do not require an internal reference dye (e.g. ROX) for normalization of fluorescent signal. Check Your cycler!

Reagents

5x One-step Multiplex Probe Mix with HOT FIREPol® DNA polymerase, reaction buffer, dNTPs including dUTP, 15 mM MgCl2 (1x qPCR solution - 3 mM MgCl2)

40x One-step SOLIScript® Mix with SOLIScript® Reverse Transcriptase, RiboGrip™ RNase Inhibitor

Water, nuclease free

Excellent for 4-plex assays
Related Resources

FAQ

Does RiboGrip help with inhibitor resistance?

RiboGrip™ is not directly associated with tolerance to common inhibitors, however, it helps to keep RNA intact because RiboGrip inhibits Rnase H.

Why do certain kits contain a ROX?

ROX (carboxy-X-rhodamine) is one of the passive reference dyes providing a constant fluorescent signal that is used for signal normalization during the amplification cycles. The emission recorded from a reference dye during the baseline cycles is used to normalize the emission recorded from the reporter (e.g. EvaGreen®, SolisGreen®, SYBR® Green, etc.) in later cycles in ROX-dependent real-time PCR systems (e.g. Applied Biosystems 5700, 7000, 7300, 7700, 7900HT, StepOne™, StepOnePlus™). Reference dye compensates for small fluorescent fluctuations and well-to-well variations that may occur.

In Solis BioDyne qPCR mixes we use technology, based on ROX, that allows us to use same mix for high- and low- ROX requiering cyclers. 

Please check cycler-mix compatibility here if not sure.

What are the storage conditions and stability of Solis BioDyne reagents?

Solis BioDyne products should be stored at -20°C.

Shipping and temporary storage for up to 1 month at room temperature (*1525°C) has no detrimental effects on the quality of Solis BioDyne reagents.

Freeze-thaw stability is tested for each product. Most PCR and qPCR products have passed 30 freeze-thaw cycles with no changes in performance. Specific information is found in Storage and Shipping conditions of each product.

When stored and handled under the recommended conditions, full activity of the reagents is retained until the Expiry Date printed on the tube label.

*World Health Organization (2003). Guidelines for the Storage of Essential Medicines and Other Health Commodities.

Storage & Shipping
Storage

Routine storage: -20 ºC
Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.

Shipping

At room temperature