Some applications of this product may require a license which is not provided by the purchase of this product.
For research use only.
Genetically modified MMLV-based reverse transcriptase with increasedthermostability and improved performance at elevated temperatures
FIREScript® Reverse Transcriptase (RT) is a genetically engineered MMLV (Moloney Murine Leukemia Virus) based Reverse Transcriptase.
This is an RNA‐directed DNA polymerase that can synthesize a complementary DNA strand from ssRNA or ssDNA and is active over a broad range of reaction temperatures from 37°C to 60°C.
FIREScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a functional RNase H domain which can increase the sensitivity of RT‐qPCR (quantitative reverse transcription PCR).
NB! A component of this kit has changed. RiboGrip® RNase Inhibitor (Cat. No. 06-22) has been discontinued and is replaced by a new product: RiboGrip® RNase Inhibitor 220 U/µl. Please refer to the product data sheet for up-to-date usage instructions.
RiboGrip™ is not directly associated with tolerance to common inhibitors, however, it helps to keep RNA intact and prevent RNA degradation because RiboGrip is inactivating ribonuclease enzymes
Pipetting viscous liquids like glycerol with an air displacement micropipette is difficult, because viscous liquids have a high resistance to flow. We have a few recommendations for accurate pipetting:
Solis BioDyne products should be stored at -20°C.
Shipping and temporary storage for up to 1 month at room temperature (*15−25°C) has no detrimental effects on the quality of Solis BioDyne reagents.
Freeze-thaw stability is tested for each product. Most PCR and qPCR products have passed 30 freeze-thaw cycles with no changes in performance. Specific information is found in Storage and Shipping conditions of each product.
When stored and handled under the recommended conditions, full activity of the reagents is retained until the Expiry Date printed on the tube label.
*World Health Organization (2003). Guidelines for the Storage of Essential Medicines and Other Health Commodities.
Routine storage: -20 ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.
At room temperature.
Robust and reliable hot-start Master Mix with higher fidelity and longer amplification range for more demanding reactions. Includes two tracking dyes.
FIREScript® KIT contains a powerful genetically modified MMLV-based FIREScript® reverse transcriptase with increased thermostability and exceptional stability at room temperature. The kit format contains only the enzyme and reaction buffer allowing you to choose other components according to your preference.
All-inclusive and the most convenient product format for first-strand cDNA synthesis. A combined enzyme mix (FIREScript® and RiboGrip®) and a reaction premix in separate tubes allowing you to choose the most appropriate priming option.
SOLIScript® KIT contains a unique in silico-designed SOLIScript® reverse transcriptase with high thermostability and exceptional stability at room temperature. SOLIScript® suits best with oligo dT and gene-specific primers. The kit format contains only the enzyme and reaction buffer allowing you to choose other components according to your preference.
