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FIREScript® KIT

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Some applications of this product  may require a license which is not provided by the purchase of this product.

For research use only. 

Fast and robust reverse transcriptase for wide range of applications

Genetically modified MMLV-based reverse transcriptase with increased
thermostability and improved performance at elevated temperatures

  • high specificity and yield
  • wide reaction temperature from 37°C to 60°C
  • fast 15 min reaction time
  • a simple 2-vial kit with enzyme and reaction buffer only
  • reaction set-up and shipment at without ice

Ordering

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Catalog number

06-13-00050

Quantity

€ 80.00

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Details

Reagents Provided
Item Pcs. Vial size
FIREScript® Reverse Transcriptase 1 10000 U | 50 µl
10x RT Reaction Buffer with DTT 1 125 µl
Overview

Description

FIREScript® Reverse Transcriptase (RT) is a genetically engineered MMLV (Moloney Murine Leukemia Virus) based Reverse Transcriptase.

This is an RNA‐directed DNA polymerase that can synthesize a complementary DNA strand from ssRNA or ssDNA and is active over a broad range of reaction temperatures from 37°C-60°C.

FIREScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a functional RNase H domain which can increase the sensitivity of RT‐qPCR (quantitative reverse transcription PCR).

Applications

  • First-strand cDNA synthesis
  • RT-PCR
  • RT-qPCR

Properties

Sample type: RNA

Final product: cDNA (first strand)

Product format: Enzyme with buffer

Enzyme: FIREScript® Reverse Transcriptase

Source: Purified from an E.coli strain that carries an overproducing plasmid containing a FIREScript® Reverse Transcriptase gene.

Unit description: One unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-precipitable material in 10 minutes at 37°C using poly(A)•oligo(dT) as a template in a total reaction volume of 50 μl.

Storage and dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.

Reagents

FIREScript® Reverse Transcriptase (200 U/μl)

10x RT Reaction Buffer with DTT (500 mM Tris-HCl pH 8.3, 500 mM KCl, 30 mM MgCl2, 100 mM DTT)

Highly competitive enzyme
Exceptional stability
cDNA synthesis priming options
Related Resources

FAQ

Enzymes are usually very viscous liquids. What would you recommend for the most accurate pipetting?

Pipetting viscous liquids like glycerol with an air displacement micropipette is difficult, because viscous liquids have a high resistance to flow. We have a few recommendations for accurate pipetting:

  • Hold the pipette vertical, since more liquid gets picked up into the tip when the pipette is held at an angle.
  • Only put the pipette tip in the liquid.
  • Use a very slow aspiration speed, wait for a few seconds until the liquid retightens and then use a very slow dispensing speed.
What are the storage conditions and stability of Solis BioDyne reagents?

Solis BioDyne products should be stored at -20°C.

Shipping and temporary storage for up to 1 month at room temperature (*15−25°C) has no detrimental effects on the quality of Solis BioDyne reagents.

Freeze-thaw stability is tested for each product. Most PCR and qPCR products have passed 30 freeze-thaw cycles with no changes in performance. Specific information is found in Storage and Shipping conditions of each product.

When stored and handled under the recommended conditions, full activity of the reagents is retained until the Expiry Date printed on the tube label.

*World Health Organization (2003). Guidelines for the Storage of Essential Medicines and Other Health Commodities.

Storage & Shipping
Storage

Routine storage: -20ºC

Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.

Shipping

At room temperature