Some applications of this product may require a license which is not provided by the purchase of this product.
For research use only.
SolisFAST® 1-step RT-PCR Kit with UNG provides a simple and streamlined workflow for analyzing RNA targets using reverse transcription PCR (RT-PCR). Both the cDNA synthesis and PCR are conveniently performed in one tube greatly simplifying the analysis workflow, reducing reaction-to-reaction variation, minimizing hands-on steps and reducing reaction set-up to results time.
Exceptionally fast and robust SolisFAST® polymerase combined with our highly processive reverse transcriptases make SolisFAST® 1-step RT-PCR Kit with UNG the fastest kit on the market to perform RT-PCR with unparalleled under 1-hour reaction times. Optimized composition ensures superior sensitivity allowing detection of as little as 0.1 pg of RNA and enabling efficient multiplexing of up to 5 RNA targets simultaneously.
Combination of our highly thermostable FIREScript® and SOLIScript® reverse transcriptases allow efficient reactions at temperatures up to 60 °C to facilitate amplifying difficult RNA targets with challenging secondary structures. The RT mix is preblended with our in silico-designed ribonuclease inhibitor RiboGrip® to minimize the risk of RNA degradation. Salini UNG® Uracil-N-Glycosylase is incorporated into the product along with dUTPs to prevent carryover contamination and false positive results.
The kit is supplied with a novel RT-PCR enhancer – AmpliBoostTM to further improve sensitivity and performance with GC-rich and other challenging amplicons. Ready-to-Load variant comes with inert loading dyes included to directly load the samples on the gel to minimize steps and save time during analysis.
Additionally, the kit is room temperature stable for up to 1 month due to the use of Stability TAG - Solis BioDyne’s proprietary and patented polypeptide stabilization technology that is incorporated into each enzyme, enabling environmentally friendly ambient temperature shipping [1].
[1] Kahre, O. et al., Compositions for increasing polypeptide stability and activity, and related methods, EP2501716B1 (2015) and US9321999B2 (2016)
SolisFAST® 1-step RT-PCR Kit with UNG is suitable for RT-PCR applications such as:
Sample type: RNA
Product format: reagent kit
Detection type: end-point analysis
Priming method: gene specific primers
Ready-to-load: no
Amplicon Size: up to 2 kb
Targets: 1-5 targets
4x One-step HOT SolisFAST® Mix with UNG with HOT SolisFAST® DNA polymerase, optimized reaction buffer, dNTPs including dUTP, 6.4 mM MgCl2 (1x qPCR solution – 1.6 mM MgCl2)
40x One-step RT Mix with SOLIScript® Reverse Transcriptase, FIREScript® Reverse Transcriptase, RiboGrip® RNase Inhibitor, Salini UNG® Uracil-N-Glycosylase.
5x AmpliBoost™ RT-PCR enhancer – A novel RT-PCR enhancer to improve sensitivity and help with challenging templates (e.g. GC-rich, secondary RNA structures).
Water, nuclease free
Green loading dye1: Mix of blue and yellow dye to allow tracking of DNA during electrophoresis.
1The mix is not recommended for use in applications where spectrophotometric measurements are necessary (e.g. Sanger sequencing). Migration speed: blue dye – TBE: ~3.5 kb, TAE: ~4.5 kb; yellow dye – TBE: ~35 bp, TAE: ~45 bp).
Routine storage: -20 ºC.
Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.
At room temperature.
Sensitive and highly specific cDNA synthesis and end-point PCR in a single tube.
