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Some applications of this product  may require a license which is not provided by the purchase of this product.

For research use only. 

Simple and highly specific cDNA synthesis and qPCR in a single tube

Convenient kit format for performing highly specific cDNA synthesis and probe-based qPCR in a single tube. The kit contains qPCR mix, SOLIScript® RT enzyme mix with RiboGrip™ RNase inhibitor, and nuclease-free water.

  • cDNA synthesis up to 60°C for superior specificity
  • suitable for singleplex and duplex assays
  • reaction set-up and shipment without ice
  • high specificity and sensitivity
  • one kit for all cyclers
  • RNase inhibitor included in kit

Ordering

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Catalog number

08-57-00250

Quantity

€ 450.00

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Details

Reagents Provided
Item Pcs. Vial size
40x One-step SOLIScript® Mix 1 250 rxn | 125 µl
5x One-step Probe Mix 1 250 rxn | 1 ml
Water, nuclease free 1 5 ml
Overview

Description

SOLIScript® 1-step Probe Kit is optimized for probe based one-step RT real-time quantitative PCR assays.

It contains all the components necessary (except template, primers, and probes) to perform cDNA synthesis and probe based qPCR in a single tube

Applications

  • Detection and quantification of RNA targets with qPCR
  • DNA/LNA hydrolysis probe based assay
  • Suitable for singleplex or duplex reactions

Properties

Sample type: RNA

Product format: Reagent kit 

No of tubes: three tubes

Detection type: Probe-based

Reference dye: reference dye based on ROX

Compatible real-time instruments: The  Kit is compatible with most qPCR cyclers that do or do not require an internal reference dye (e.g. ROX) for normalization of fluorescent signal. Check Your cycler!

Reagents 

5x One-step Multiplex Probe Mix (ROX) with HOT FIREPol® DNA polymerase, reaction buffer, dNTPs including dUTP), 15 mM MgCl2 (1x qPCR solution - 3 mM MgCl2), internal reference based on ROX dye

40x One-step SOLIScript® Mix with SOLIScript® Reverse Transcriptase and RiboGrip™ RNase Inhibitor

Water, nuclease free

Excellent quantification in duplex assays
Related Resources

FAQ

Does RiboGrip help with inhibitor resistance?

RiboGrip™ is not directly associated with tolerance to common inhibitors, however, it helps to keep RNA intact because RiboGrip inhibits Rnase H.

Why do certain kits contain a ROX?

ROX (carboxy-X-rhodamine) is one of the passive reference dyes providing a constant fluorescent signal that is used for signal normalization during the amplification cycles. The emission recorded from a reference dye during the baseline cycles is used to normalize the emission recorded from the reporter (e.g. EvaGreen®, SolisGreen®, SYBR® Green, etc.) in later cycles in ROX-dependent real-time PCR systems (e.g. Applied Biosystems 5700, 7000, 7300, 7700, 7900HT, StepOne™, StepOnePlus™). Reference dye compensates for small fluorescent fluctuations and well-to-well variations that may occur.

In Solis BioDyne qPCR mixes we use technology, based on ROX, that allows us to use same mix for high- and low- ROX requiering cyclers. 

Please check cycler-mix compatibility here if not sure.

What are the storage conditions and stability of Solis BioDyne reagents?

Solis BioDyne products should be stored at -20°C.

Shipping and temporary storage for up to 1 month at room temperature (*1525°C) has no detrimental effects on the quality of Solis BioDyne reagents.

Freeze-thaw stability is tested for each product. Most PCR and qPCR products have passed 30 freeze-thaw cycles with no changes in performance. Specific information is found in Storage and Shipping conditions of each product.

When stored and handled under the recommended conditions, full activity of the reagents is retained until the Expiry Date printed on the tube label.

*World Health Organization (2003). Guidelines for the Storage of Essential Medicines and Other Health Commodities.

Storage & Shipping
Storage

Routine storage: -20 oC

Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of SOLIScript® 1-step Probe Kit

Shipping

At room temperature