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For research use only.
For loading DNA samples like PCR fragments and restriction fragments on gel.
6x DNA Loading Dye Buffer Orange and Blue is used for loading DNA samples (PCR products, restriction fragments) on agarose or polyacrylamide gel.
The loading dye serves as visual marker of electrophoresis: in 1% agarose gel Orange G comigrates with ca 40 bp (1x TBE) or ca 60 bp (1x TAE) fragments and Xylene Cyanole FF comigrates with ca 3500 bp (1x TBE) or ca 5000 bp (1x TAE) fragments.
Concentration: 6x
Reagent Composition: Orange G, Xylene Cyanole FF, TRIS-HCl, EDTA and Ficoll
Recommendations:Mix 1 volume of Orange and Blue Dye with 5 volumes of your DNA sample and load into the gel.
The 6X Loading Dye buffer is used to prepare DNA ladders and samples for loading on agarose or polyacrylamide gels.
Loading Dye Buffers contain glycerol that ensures that the DNA in the ladder and sample forms a layer at the bottom of the well, and color markers (e.g. Bromophenol blue, Xylene cyanole FF, Orange G) for visual tracking of DNA migration during electrophoresis.
The concentration of agarose in a gel depends on the sizes of the DNA fragments, and should be able to separate your fragment(s) enough so that they are identifiable.
Smaller fragments of DNA migrate through the gel more quickly and, therefore, are separated on higher concentrations of agarose. Larger molecules require a lower concentration of agarose.
Dilute your DNA sample with the 6x DNA Loading Dye Buffer: mix 1 volume of the Loading Dye Buffer with 5 volumes of your DNA sample.
For the best results, use Solis BioDyne 6x Loading Dye Buffers (Blue Dye Cat. No. 07-01-00010, Double Blue Dye 07-02-00010, Orange Dye 07-04-00010, Orange Blue Dye 07-03-00010).
Store at room temperature or at 4ºC.Long-term storage at -20ºC.
At room temperature
