The running joke with PCR is that if something can go wrong, it will go wrong. Quite often it’s even impossible to determine why some samples turned out fine while the others did not. In a situation like this, it would be amazing to know some trick or a secret to avoid spending all the time and resources to do the experiment again. Here are a few we are willing to share so that you could find love for PCR.

There are two options for target detection when doing qPCR - either you use dye or probe-based detection. Today we are going to take a deep dive into the world of dye-based qPCR, which was invented during the early nineties by Russell Higuchi [1] and is still used daily around the world.

Science is continually moving toward more accurate and individual-specific approaches when it comes to developing new genotyping technologies. In light of that, we would like to discuss high-throughput genotyping, which allows for a large number of precise results to be obtained fast.

Contamination can ruin your day or even week. Data is not valid, a lot of cleaning must be done and supplies are wasted. Precious time and even samples can be lost. Prevention is the best strategy!