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Endpoint PCR Enzymes and Master Mixes
Product Hot Start Ready to Load dUTP+ UNG Fidelity vs. Taq Amplification Rangea Resulting ends Speed GC-rich performance Multiplex PCR
FIREPol® DNA Polymerase Kit 1x 5 kb 3’A * ** **
FIREPol® Master Mix 1x 5 kb 3’A * * *
FIREPol® Master Mix Ready to Load • 1x 5 kb 3’A * * *
HOT FIREPol® DNA Polymerase Kit • 1x 5 kb 3’A * ** **
HOT FIREPol® GC Master Mix Kit • 1x 5 kb 3’A * *** *
HOT FIREPol® MultiPlex Mix • 1x 5 kb 3’A * * ***
HOT FIREPol® MultiPlex Mix Ready To Load • • 1x 5 kb 3’A * * ***
HOT FIREPol® Blend Master Mix • 5x 5 kb 3’A/Blunt * * *
HOT FIREPol® Blend Master Mix Ready to Load • • 5x 5 kb 3’A/Blunt * * *
NEW! SolisFAST® Master Mix • 1x 5 kb 3’A *** * ***
NEW! SolisFAST® Master Mix, Ready to Load • • 1x 5 kb 3’A *** * ***
NEW! SolisFAST® Master Mix with UNG • • 1x 5 kb 3’A *** * ***
NEW! SolisFAST® Master Mix with UNG, Ready to Load • • • 1x 5 kb 3’A *** * ***

a Enables amplification of up to 5 kb fragments from low complexity DNA templates (e.g. cDNA, lambda, plasmid DNA), and up to 3 kb from genomic DNA (human, animal, plant).

qPCR Cycler Compatibility: dye-based and probe-based mixes
Following trademarks are registered by following companies: Applied Biosystems and StepOne are trademarks of Applied Biosystems LL C. QuantStudio, ViiA and Mustang Purple are trademarks of Life Technologies Corporation. Stratagene, Mx3000P, Mx3005P and Mx4000 are trademarks of Agilent Technologies inc. Mastercycler is a trademark of Eppendorf AG . Rotor-Gene is a trademark of Qiagen Group. Bio-Rad, CFX96, CFX384, iQ,MyiQ, Opticon 2, Chromo4, MiniOpticon are the trademarks of Bio-Rad Laboratories. LightCycler and TaqMan are trademarks of Roche Molecular Systems Inc. The Eco is a trademark of Illumina Inc. PikoReal is a trademark of Thermo Fisher Scientific Inc. EvaGreen is a trademark of Biotium Inc..
Mixes for dye-based qPCR assays
Product Speed Sensitivity GC-rich performance dUTP UNG
NEW! SolisFAST® SolisGreen® qPCR Mix *** ** *
HOT FIREPol® SolisGreen® qPCR Mix 2.0 * *** *
HOT FIREPol® EvaGreen® qPCR Supermix * ** *** •
HOT FIREPol® EvaGreen® qPCR Mix Plus * * *
HOT FIREPol® EvaGreen® HRM Mix * ***

Mixes for probe-based qPCR assays
Product Speed GC-rich performance Multiplex qPCR dUTP UNG
NEW! SolisFAST® Probe qPCR Mix *** * up to 5 targets
NEW! SolisFAST® Lyo-Ready qPCR Kit with UNG *** * up to 5 targets
NEW! SolisFAST® Probe qPCR Mix with UNG *** * up to 5 targets • •
HOT FIREPol® Multiplex qPCR Mix * *** up to 4 targets •
HOT FIREPol® Probe Universal qPCR Kit * *** up to 2 targets •
HOT FIREPol® Probe qPCR Mix Plus * * up to 2 targets

Solis BioDyne’s probe-based qPCR mixes are optimized for real-time quantitative PCR assays and contain all the components necessary (except primers and probes) to perform qPCR. Probe-based qPCR is based on 5' flap endonuclease activity and our mixes are optimized for DNA/LNA hydrolysis probes (i.e. TaqMan probes)

To find a suitable mix for your qPCR cycler please check our qPCR Cycler Compatibility tool

First Strand cDNA synthesis
Product Specification Product  format Reaction temperature RNase H activity
NEW! FIREScript® RT cDNA synthesis MIXExcellent enzyme for standard applications
  • 3-vial kit format
  • 4 priming optionsa
 37°C -60°C Full
FIREScript® RT cDNA synthesis KIT
  • 7-vial kit format
  • 4 priming optionsa
 37°C -60°C Full
FIREScript® KIT
  • enzyme and reaction buffer only
 37°C -60°C Full
SOLIScript® RT cDNA synthesis MIXSuperior specificity with complex templates and specific primers
  • 3-vial kit format
  • primers not includedb
 37°C -60°C Reduced
SOLIScript® RT cDNA synthesis KIT
  • 6-vial kit format
  • oligo (dT) primers includedc
 37°C -60°C Reduced
SOLIScript® KIT
  • enzyme and reaction buffer only
 37°C -60°C Reduced

a Primers provided with the product: oligo (dT), random, oligo (dT) and random combined. Gene-specific primers to be supplied by the user.

b Gene-specific or oligo (dT) primers to be supplied by the user. SOLIScript® RT is not compatible with random primers. 

c Gene-specific primers to be supplied by the user. SOLIScript® RT is not compatible with random primers. 

One-step RT-(q)PCR
  Product No. of targets per reactions GC-rich performance dUTP UNG Passive reference dye Compatible cyclers Incompatible probe reporter dyes
Dye-based detectionSOLIScript® 1-step SolisGreen® Kit 2.01 * • ROX Most cyclers, except ABI StepOne and StepOnePlus, 7300, 7900HT n/a
Probe-based detectionSOLIScript® 1-step Probe Kit1-2 * ROX All cyclers ROX, JUN, Texas Red
SOLIScript® 1-step Multiplex Probe Kit | Without ROX1-4 *** • None Most cyclers, except ABI and Stratagene
SOLIScript® 1-step Multiplex Probe Kit | ROX1-4 *** • ROX All cyclers. Recommended with ABI and Stratagene ROX, JUN, Texas Red
SOLIScript® 1-step Multiplex Probe Kit | Purple1-4 *** • Purple ABI cyclers with Mustang Purple® detection channel Cy5
NEW! SOLIScript® Fast 1-step RT-qPCR Mix with UNG1-5 ** • • None Most cyclers