Some applications of this product may require a license which is not provided by the purchase of this product.
For research use only.
Fast and sensitive glycerol-free qPCR Mix and a carefully optimized lyophilization excipient mix for effective freeze-drying to cakes or beads.
SolisFAST® Lyo-Ready qPCR Kit with UNG is an optimized solution for fast and sensitive amplification and quantitation of DNA targets with probe-based assays. The kit comes in 2 tubes: the glycerol-free 5x SolisFAST® Lyo-compatible qPCR Mix with UNG and 4x SolisFAST® Lyo Excipient Mix. The composition is optimized for lyophilization to cakes or beads.
5x SolisFAST® Lyo-compatible qPCR Mix with UNG is a 5x concentrated qPCR master mix suitable for real-time detection and quantitation of up to five DNA targets simultaneously. It contains dUTPs instead of dTTPs, as well as Salini UNG® Uracil-N-Glycosylase to prevent carry-over contamination from previous amplifications. The mix does not contain any lyophilization additives or excipients.
4x SolisFAST® Lyo Excipient Mix is a 4x concentrated blend of additives that protect reagents during lyophilization. In combination with the glycerol-free qPCR mix, it enables lyophilization to beads or cakes.
Sample type: DNA
Hot-start: yes, 30 sec to 3 min initial activation at 95 °C
Cycling speed: fast or ultra-fast
Product format: reagent kit
Detection type: probe-based
Reference dye: none
Compatible real-time instruments: The product is compatible with most qPCR cyclers. If using a ROX-dependent platform, add ROX in concentrations recommended by the platform manufacturer, or switch off ROX normalization prior to the run.
SolisFAST® Lyo-Ready qPCR Kit with UNG comprises:
SolisFAST® DNA polymerase, Salini UNG® Uracil-N-Glycosylase, nucleotides (dUTPs, dATPs, dCTPs and dGTPs), MgCl2 (5x mix - 16.5 mM, 1x mix – 3.3 mM), glycerol-free reaction buffer.
A proprietary mix of lyophilization additives that serve as cryoprotectants and stabilizers.
Please click HERE to open the Data Sheet of SolisFAST® Lyo-Ready qPCR Kit with UNG or find it under Related Resources. The Data Sheet includes the recommended lyophilization protocol.
Yes, it is possible to opt for your own set of lyophilisation excipients with the SolisFAST® Lyo-compatible qPCR Mix with UNG. However, for the most optimal lyophilisation conditions and in order to achieve the most stable lyophilizates, we highly recommend using the dedicated SolisFAST® Lyo Excipient Mix.
Yes, it is possible to use this mix for liquid assays. However, if lyophilization is not desired, we recommend using our dedicated master mixes for regular liquid assays. The closest analogue to SolisFAST® Lyo-Ready qPCR Kit with UNG is SolisFAST® Probe qPCR Mix with UNG, catalog number 28-21-0001.
To protect the biological material, such as enzymes, from the temperature and pressure changes during lyophilization, freeze-drying protectants are employed. These protecting excipients ensure that the materials reserve their active state after lyophilisation. In addition to that, they provide an adequate structure to the lyophilized cake or bead.
Lyophilization, commonly known as freeze-drying, is a process employed to remove the water content from a substance by sublimation after it has been frozen, while preserving its structure and properties. The reagents can be lyophilized to a spheric lyo-bead or a compact lyo-cake format.
Lyophilization helps to extend the product’s shelf life, enhance the stability of the reagents at higher tempereatures, even when mixed together with primers and probes, and allows for convenient and accurate dispensing of the reagents. After lyophilization, the reagents are easily reconstituted with sample material.
Routine storage: -20 °C
Temporary storage for up to 2 weeks at room temperature has no detrimental effects on the quality of SolisFAST® Lyo-Ready qPCR Kit with UNG.
On ice packs.
NB! Due to the use of ice packs, the shipping cost is approximately 200 euros (depending on package size and destination country).
RiboGrip® RNase Inhibitor is an in silico-designed protein-based ribonuclease inhibitor with enhanced stability. Unique high concentration and glycerol free formulation is ideal for lyophilization.
Exceptionally stable SoliSD™ Lyo-compatible Bsm DNA Polymerase for isothermal amplification.
In the new SoliSD™ Lyo-compatible RT-LAMP Kit all enzymes are compatible with freeze drying and contain the Stability TAG.
