Some applications of this product may require a license which is not provided by the purchase of this product.
For research use only.
Find out more from the webinar HERE.
Salini UNG® Uracil-N-Glycosylase is a unique heat-labile enzyme. The protein sequence originates from the bacteria genus Salinivibrio which is frequently found in hypersaline environments. Uracil-N-Glycosylase (UNG) efficiently eliminates uracil from single- or doublestranded DNA by catalyzing the hydrolysis of the N-glycosylic bond and leaving an abasic site. This property is widely used as a part of PCR carryover contamination prevention strategy. Salini UNG® is a genetically modified enzyme including a Stability TAG - Solis BioDyne’s proprietary and patented polypeptide stabilization technology that makes all our proteins extremely stable at room temperature [1].
Concentration: 1 U/µl
Source: Purified from an E.coli strain that carries an overproducing plasmid containing a Salini UNG® Uracil-N-Glycosylase gene.
Storage and dilution buffer: 50% glycerol (v/v), 25 mM Tris-HCl pH 7.5, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT and stabilizers.
Working concentration: For qPCR application we recommend to use Salini UNG® Uracil-N-Glycosylase at a final concentration of 0.025-0.04 U/μl, for endpoint PCR application at a final concentration of 0.005-0.01 U/μl.
Reaction temperature: If you setup reaction at room temperature, no additional treatment step is required. If you setup reaction on ice add UNG treatment step prior amplification: 30 seconds at 25 °C. Working temperature 25-40 °C.
Inactivation: 5 min at 70 °C.
Routine storage: -20 ºC.
At room temperature.
Ultra fast endpoint PCR Master Mix that includes novel in-silico designed SolisFAST® DNA Polymerase for robust and accurate target detection. Suitable for UNG treatment to prevent carryover contamination.
Ultra fast endpoint PCR Master Mix that includes novel in-silico designed SolisFAST® DNA Polymerase for robust and accurate target detection. Suitable for UNG treatment to prevent carryover contamination. Includes two tracking dyes.
Highly specific and reproducible dye-based qPCR Mix with blue visualization dye for easy pipetting. Suitable for ROX-dependent and ROX-independent qPCR cyclers.
High-performing probe-based qPCR Mix for AT-rich, regular and GC-rich templates.
Probe-based qPCR Mix optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays
Ultra-fast probe-based qPCR Master Mix that includes novel in-silico designed SolisFAST® DNA Polymerase for robust and accurate target quantification. Suitable for UNG treatment to prevent carryover contamination.
Sensitive and highly specific cDNA synthesis and probe-based qPCR in a single tube. Ideal solution for high throughput RNA analysis.
