Some applications of this product may require a license which is not provided by the purchase of this product.
For research use only.
RiboGrip™ RNase Inhibitor is an in silico-designed protein-based ribonuclease inhibitor, which inactivates RNase A, RNase B and RNase C (III).
RiboGrip™ inhibits the activity of ribonuclease A by noncovalently binding in a non-competitive mode at a ratio of 1:1. This novel inhibitor has enhanced stability at room temperature with no loss of activity for up to 1 month. Included in FIREScript® and SOLIScript® cDNA Synthesis Kits and Mixes, and in all SOLIScript® 1-step RT-PCR kits.
Preventing RNA degradation in the following applications:
Concentration: 40 U/µl
Source: Purified from an E.coli strain that carries an overproducing plasmid containing a RiboGrip™ RNase Inhibitor gene.
Storage and dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 7.5, 100 mM KCl, 0.1 mM EDTA, 5 mM DTT and stabilizers.
Recommendations for use: RiboGrip RNase Inhibitor™ is used generally at a final reaction concentration of 0.1-1 U/μl. Inactivated by heating at 85°C for 5 min.
Routine storage: -20ºC.
Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.
At room temperature.
Sensitive and highly specific cDNA synthesis and qPCR in a single tube. Suitable for ROX and no ROX cyclers.
Sensitive and highly specific cDNA synthesis and probe-based qPCR in a single tube. Ideal solution for high throughput RNA analysis.
Highly sensitive cDNA synthesis and dye-based qPCR in a single tube. Ideal solution for high throughput RNA analysis.
FIREScript® KIT contains a powerful genetically modified MMLV-based FIREScript® reverse transcriptase with increased thermostability and exceptional stability at room temperature. The kit format contains only the enzyme and reaction buffer allowing you to choose other components according to your preference.
A flexible and robust cDNA synthesis kit contains all the reagents you need for first-strand cDNA synthesis. Individual components enable thorough assay optimization.
All-inclusive and the most convenient product format for first-strand cDNA synthesis. A combined enzyme mix (FIREScript® and RiboGrip™) and a reaction premix in separate tubes allowing you to choose the most appropriate priming option.
SOLIScript® KIT contains a unique in silico-designed SOLIScript® reverse transcriptase with high thermostability and exceptional stability at room temperature. SOLIScript® suits best with oligo dT and gene-specific primers. The kit format contains only the enzyme and reaction buffer allowing you to choose other components according to your preference.
A flexible and highly specific cDNA synthesis kit that contains all the reagents you need for first-strand cDNA synthesis. Individual components enable thorough assay optimization. Note! The kit includes oligo dT primers, SOLIScript® is not recommended with random primers.
All-inclusive and the most convenient product format for first-strand cDNA synthesis using gene-specific primers. A combined enzyme mix (SOLIScript® and RiboGrip™) and a reaction premix in separate tubes. Primers not included.
