Technology

    

All enzymes produced at Solis BioDyne are exceptionally stable at room temperature due to a specific genetic modification - Stability TAG. Stability TAG increases polypeptide shelf life and its tolerance for temperature at a wider scale without compromising the properties of the polypeptide itself. After being stored at room temperature for one month, there is no detectable change in the performance of the enzymes.

Stability TAG

Stability TAG Technology is now applied in all Solis BioDyne's products providing the client with enzymes, end-point Master Mixes and qPCR Mixes with exceptional stability at room temperature.

Tested on a variety of proteins

The stabilizing effect of Stability TAG has been tested on a variety of proteins including Taq DNA Polymerase, reverse transcriptase, RNase inhibitor, hEPO, PCNA, pyrophosphatase and hLIF.

Stability TAG does not affect the properties of the protein

A number of tests with different Stability TAG attached proteins revealed no adverse effect on protein properties. For example, pluripotency was maintained in ES cells during gene targeting using Stability TAG-hLIF; amplification of DNA using Stability TAG-Taq DNA Polymerase (FIREPol® DNA Polymerase) was performed with the same efficiency and accuracy as with regular Taq.

Storage at room temperature does not affect enzyme performance

All our products have been tested for stability at room temperature for an extended time. For illustration, the qPCR performance of a product that was stored at -20°C was compared to the same product stored at room temperature for 4 weeks. There is no detectable difference in the performance of 5x HOT FIREPol® Probe Universal qPCR Mix (Figure A) or FIREScript® Reverse Transcriptase (Figure B) after 4 weeks of storage at room temperature.

Long term stability at -20ºC

In addition to excellent stability at ambient temperature, enzymes produced at Solis BioDyne also exhibit extraordinarily long shelf life. Even after 18 years, our first batch of FIREPol® DNA Polymerase shows satisfactory results in PCR.

Patented technology

Patent EP2501716, "Compositions for increasing polypeptide stability and activity, and related methods", is now granted in the EU. Patent is published in European Patent Bulletin 21/04/15!