Solis BioDyne | Product Selection Guides

Endpoint PCR Enzymes and Master Mixes

Product	Hot Start	Ready to Load	dUPT+ UNG	Fidelity vs. Taq	Amplification Range^a	Resulting ends	Speed	GC-rich performance	Multiplex PCR
FIREPol^® DNA Polymerase	•			1x	5 kb	3'A	*	***	**
NEW! 5x FIREPol^® Master Mix Ready to Load		•		1x	5 kb	3'A	***	**	*
5x FIREPol^® Master Mix	•		•	5x	5 kb	3'A	*	*	*

Mix compatimbility table

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FIREPol is a trademark of Solis BioDyne. Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat. FIREPol is a trademark of Solis BioDyne. Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat.

Mixes for dye-based qPCR assays

Product	Speed	Sensivity	GC-rich performance	dUPT	UNG
NEW! 5x HOT FIREPol^® SolisGreen qPCR Mix	***	*	**	•

Mixes for probe-based qPCR assays

Product	Speed	GC-rich performance	Multiplex qPCR	dUPT	UNG
5x HOT FIREPol^® Probe qPCR Mix Plus	***	*	≤ 5 targets	•

First Strand cDNA synthesis

Product	Specification	Product format	Reaction temperature	RNase H activity
FIREScript RT cDNA synthesis MIX	Excellent enzyme for standard applications	3-vial-kit format 4 priming optoins^a	37°C-60°C	Full
NEW! FIREScript RT cDNA synthesis KIT		7-vial-kit format 4 priming optoins^a	37°C-60°C	Full
FIREScript KIT		enzyme and reaction buffer only	37°C-60°C	Full
SOLIScript RT cDNA synthesis MIX	Superior specificity with complex templates and specific primers	3-vial-kit format gene-specific primers only^b	37°C-60°C	Reduced
NEW! SOLIScript RT cDNA synthesis KIT		6-vial-kit format 2 priming options^c	37°C-60°C	Reduced
SOLIScript KIT		enzyme and reaction buffer only	37°C-60°C	Reduced

One-step RT-(q)PCR

	Product	Nr of targets per reaction	GC-rich performance	dUPT	UNG	Passive reference dye	Compatible cyclers	Incompatible probe reporter dyes
Dye-based detection	5x HOT FIREPol^® SolisGreen qPCR Mix	1	*	•		Purple		Cy5
Endpoint detection	NEW! FIREPol^® DNA Polymerase					ROX
Probe-based detection	SOLIScript 1-step Probe Kit	1-2	*		•	ROX	All cyclers	ROX JUN Texas Red
Probe-based detection	NEW! SOLIScript 1-step Multiplex Probe Kit	1-4	***	•		None	All cyclers except Applied BioSystems and Agilent

high sensitivity and specificity excellent efficiency reaction set-up and shipment without dry ice cost-effective solution for wide range of applications

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