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For research use only.
Convenient kit format for performing highly specific cDNA synthesis and probe-based qPCR in a single tube. The kit contains qPCR mix, SOLIScript® RT enzyme mix with RiboGrip™ RNase inhibitor, and nuclease-free water.
SOLIScript® 1-step CoV Kit is optimized for highly sensitive detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral RNA by one-step real-time RT-PCR (RT-qPCR).The Kit is suitable for the detection of up to four targets simultaneously and can be used with all common qPCR cyclers. Follow recommendations specific for a selected SARS-CoV-2 diagnostic panel, and instrument-specific guidelines. Contact us if a passive reference dye is required for signal normalization.SARS-CoV-2 primers and probes, applicable positive and internal extraction controls are not provided with the Kit and should be supplied by the user. If full solution is needed, please check out: SOLIScript® SARS-CoV-2 RT-qPCR Multiplex Assay Kit.
SOLIScript® 1-step CoV Kit is tested and validated for SARS-CoV-2 detection by diagnostic laboratories. Please see our recommendations for US-CDC and Berlin-Charité protocols.
Note! Amplification of more than one target in a single reaction may require considerable optimization, individual assays must be validated prior to multiplexing. For multiplex reactions, design primer pairs with similar annealing temperatures for all targets to be amplified. Self-complementarity of primers should be avoided.
Detection and quantification of RNA targets with qPCR (including SARS-CoV-2)
DNA/LNA hydrolysis probe-based assays
Suitable for singleplex and multiplex reactions
Sample type: RNA
Product format: Reagent kit
Detection type: Probe-based
Reference dye: not included
Compatible real-time instruments: Most qPCR platforms. Check Your cycler!
5x One-step Probe CoV Mix with HOT FIREPol® DNA polymerase, reaction buffer, dNTPs, 15 mM MgCl2 (1x qPCR solution - 3 mM MgCl2)
40x One-step SOLIScript® CoV Mix with SOLIScript® Reverse Transcriptase and RiboGrip™ RNase Inhibitor
Water, nuclease free
RiboGrip™ is not directly associated with tolerance to common inhibitors, however, it helps to keep RNA intact and prevent RNA degradation because RiboGrip is inactivating ribonuclease enzymes
The Kit has been tested and validated by diagnostic laboratories using primer-probe panels recommended by Charité, (Germany) and CDC (USA) protocols according to WHO “Coronavirus disease (COVID-19) technical guidance: Laboratory testing for 2019-nCoV in humans”, section: “Molecular assays to diagnose COVID-19”; subsection: “In-house developed molecular assays”
SARS-CoV-2 primers and probes, applicable positive and internal extraction controls are not provided with the Kit and should be supplied by the user.
ROX (carboxy-X-rhodamine) is one of the passive reference dyes providing a constant fluorescent signal that is used for signal normalization during the amplification cycles. The emission recorded from a reference dye during the baseline cycles is used to normalize the emission recorded from the reporter (e.g. EvaGreen®, SolisGreen®, SYBR® Green, etc.) in later cycles in ROX-dependent real-time PCR systems (e.g. Applied Biosystems 5700, 7000, 7300, 7700, 7900HT, StepOne™, StepOnePlus™). Reference dye compensates for small fluorescent fluctuations and well-to-well variations that may occur.
In Solis BioDyne qPCR mixes we use technology, based on ROX, that allows us to use same mix for high- and low- ROX requiering cyclers.
Please check cycler-mix compatibility here if not sure.
Routine storage: -20oC
Temporary storage for up to 30 days at room temperature has no detrimental effects on the quality of SOLIScript® 1-step CoV Kit
At room temperature
Sensitive and highly specific cDNA synthesis and qPCR in a single tube. Suitable for ROX and no ROX cyclers.
Sensitive and highly specific cDNA synthesis and probe-based qPCR in a single tube. Ideal solution for high throughput RNA analysis.
The SOLIScript® SARS-CoV-2 RT-qPCR Multiplex Assay Kit is an in vitro qualitative diagnostic test intended for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genetic material in biological samples obtained from the upper respiratory tract(nasopharyngeal swabs).
SOLIScript® SARS-CoV-2 RT-qPCR Multiplex Assay Kit 2.0 is a multiplex assay optimized for RT-qPCR detection of SARS-CoV-2 viral RNA extracted from clinical samples or extracted from any other sample containing SARS-CoV-2 viral RNA. Includes primers and positive control.
