All enzymes produced at Solis BioDyne are exceptionally stable at room temperature due to a specific genetic modification - Stability TAG. Stability TAG increases polypeptide self life and its tolerance for temperature at a wider scale without compromising the properties of the polypeptide itself. After being stored at room temperature for one month, there is no detectable change in the performance of the enzymes.
Stability TAG Technology is now applied in all of Solis BioDyne's products providing the client with enzymes, end-point Master Mixes and qPCR Mixes with exceptional stability at room temperature.
The stabilizing effect of Stability TAG has been tested on a variety of proteins including Taq DNA Polymerase, reverse transcriptase, RNase inhibitor, hEPO, PCNA, pyrophosphatase and hLIF.
A number of tests with different Stability TAG attached proteins revealed no affect on protein properties. For example, pluripotency was maintained in ES cells during gene targeting using Stability TAG-hLIF; amplification of DNA using Stability TAG-Taq DNA Polymerase (FIREPol® DNA Polymerase) was performed with the same efficiency and accuracy as with regular Taq.
All our products have been tested for stability at room temperature. This can be illustrated with performance comparison tests between a product that was stored at -20°C and the same product that was stored at room temperature for 4 weeks instead. There are no detectable differences in the performance of 5x HOT FIREPol® Probe Universal qPCR Mix (Figure A) or FIREScript reverse transcriptase (Figure B) after 4 weeks of room temperature storage.
In addition to excellent stability at ambient temperature, enzymes produced at Solis BioDyne also exhibit extraordinarily long shelf life. Even after 18 years, our first batch of FIREPol® DNA Polymerase gives satisfactory results in PCR (Figure C).
Figure C
